BioinformaticsBrowser-onlyFASTA-awareIUPAC support
Reverse Complement
Paste a DNA or RNA sequence, strip FASTA headers automatically, and get the complement or reverse complement in your browser.
Sequence input
Whitespace is removed automatically. Invalid characters are flagged before output is generated.
FASTA headers are ignored automaticallyDNA mode accepts A, C, G, T and IUPAC ambiguity codes.
Sequence summary
Counts are based on the cleaned sequence after whitespace and FASTA headers are removed.
Cleaned length
39
Includes recognized and invalid characters.
GC%
56.4%
Calculated from the cleaned sequence.
Valid bases
39
Recognized sequence characters only.
Headers stripped
0
FASTA headers beginning with >.
Base count summary
A
9
C
8
G
14
T
8
Ambiguous
0
Primary bases
39
Complement
Swap each base for its partner without reversing the string.
TACCGGTAACATTACCCGGCGACTTTCCCACGGGCTATC
Reverse
Reverse the cleaned sequence as-is.
GATAGCCCGTGGGAAAGTCGCCGGGTAATGTTACCGGTA
Reverse complement
Complement first, then reverse for a strand-matched sequence.
CTATCGGGCACCCTTTCAGCGGCCCATTACAATGGCCAT
How it works
A compact workflow for quick sequence checks before you move into notebooks or downstream analysis.
1
Paste a DNA or RNA sequence, including FASTA records.
2
The tool strips headers, normalizes whitespace, and checks for invalid characters.
3
Copy the complement, reverse sequence, or reverse complement with one click.
When to use it
Best for fast checks before a notebook, primer review, or protein-related workflow.
Sanity-check an oligo or amplicon before downstream work.
Prep sequence snippets for exploratory analysis in Runcell.
Move from quick sequence prep into notebook-based lab notes.
FAQ
Short answersWhat sequences can I paste here?
The tool accepts DNA or RNA sequences and supports common IUPAC ambiguity codes such as N, R, Y, S, W, K, M, B, D, H, and V.
Does the tool strip FASTA headers?
Yes. Lines that begin with > are ignored automatically, so you can paste FASTA records directly.
Does processing happen in the browser?
Yes. The sequence is normalized and reversed locally in your browser before you copy the result.
How is reverse complement different from complement?
Complement swaps each base for its partner, while reverse complement first complements the sequence and then reverses it.
Related bioinformatics tools
Move from sequence cleanup into the next step of the same bioinformatics cluster.
GC Content Calculator
Check sequence composition before downstream analysis.
Restriction Site Finder
Scan the cleaned sequence for common enzyme motifs.
DNA Concentration Calculator
Convert sequence prep inputs into common lab units.
Protein Concentration Calculator
Continue into protein-side experiment planning.
Bridge to Runcell
Use this page for quick sequence prep, then move the next step into a notebook or analysis workflow.
If the sequence is part of a bigger bioinformatics or protein analysis workflow, Runcell helps you document the follow-up steps and keep the analysis reproducible.