Restriction Site Finder
Paste a DNA sequence and scan for common restriction enzyme sites in your browser. Built for quick cloning checks, plasmid map review, and sequence prep before deeper analysis.
Search by enzyme or recognition site, then choose exactly what to scan.
See which enzymes match, where they cut, and how many sites appear in the sequence.
The sequence is normalized locally, so you can use it for quick checks without a server round-trip.
Designed to support cloning prep and the bioinformatics workflows that later move into Runcell notebooks.
The catalog is a versioned snapshot of commercially available enzymes from Biopython restriction data derived from REBASE. Recognition sites support IUPAC ambiguity codes and both DNA strands.
1. FASTA headers and whitespace are removed.
2. Lowercase input is normalized and U is converted to T.
3. The sequence is scanned on both strands against the selected enzyme motifs in the browser.
Each row shows the recognition motif, the cut pattern, the number of hits, and the 1-based start positions.
Use the copy button to move the table into notes, notebook cells, or a cloning checklist.
After a quick site check, many users move into reverse-complement, GC-content, or concentration calculations before analysis.
Flip sequence orientation before downstream checks.
Measure sequence composition and quality at a glance.
Convert DNA quantities while you prepare cloning inputs.
Continue into protein-side planning after sequence prep.
Restriction Site Finder FAQ
Answers to the most common sequence-scanning questions.