Runcell posts tagged “protein”
BCA vs Bradford: How Far Off Each Assay Reads 13 Proteins Against a BSA Standard
A protein-by-protein bias table for BCA, Micro BCA, Modified Lowry, Bradford and Bradford Plus, built from the vendor's own response ratios, plus an A280 column computed from UniProt. Shows where the assays disagree by up to 3.4×, what switching to a BGG standard fixes for antibodies, and why the Bradford numbers are a best case.Read More →
A280 Protein Concentration Tutorial: ε280 for 11 Proteins, and Why A280 ≠ mg/mL
Computed ε280, molecular weight and Abs 0.1% for 11 common lab proteins from UniProt mature chains. Shows how far the '1 A280 = 1 mg/mL' shortcut is off (0.17× to 2.65×), what pasting the precursor sequence costs, and why nucleic-acid contamination skews proteins with a low Abs 0.1% the most.Read More →