Runcell posts tagged “pcr”

Primer Dimer Check Tutorial: Testing ΔG Rules on the CDC qPCR Primers

We ran the CDC SARS-CoV-2 N1, N2, N3 and RNase P primers and probes through two dimer models and Primer3. A consecutive-pair checker at common ΔG limits misses the N3 set that CDC withdrew. Ranked by Primer3's 3′-end stability, the N3 pair comes first and two of three reported N2 interactions land in the top four. With the numbers, the code, and how to read a dimer flag.Read More →