Runcell posts tagged “cloning”

Dam/Dcm Methylation Tutorial: Which Restriction Sites Are Blocked in pUC19, pBR322, pACYC184 and Lambda

A site-by-site table of which restriction sites Dam and Dcm methylation block in four standard DNAs, for the 114 enzymes in Runcell's restriction site finder, using REBASE sensitivity data. Covers the enzymes that are on the 'blocked' list but still cut your vector, how often a cloning junction creates a new blocked site, and a script to check your own plasmid.Read More →